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_3

Full identifier: http://purl.org/np/RAysi2SPEKm8TaYscHjRtWBr9tT0a2IA9d0jhRLRDP8bc#_3

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Minted in Nanopublication

RAysi2SPEK Step comment approve/disapprove edit as derived nanopublication

http://purl.obolibrary.org/obo/CHEBI_17997

CHEBI_17997

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"liquid nitrogen"

http://purl.obolibrary.org/obo/PO_0009005

PO_0009005

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"root"

http://purl.obolibrary.org/obo/PO_0025034

PO_0025034

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"leaf"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_1

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"recalcitrant samples"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_2

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"recalcitrant samples"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_3

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"pestle"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_4

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"mortar"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_5

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"extraction buffer"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://purl.org/dc/elements/1.1/description

description

(this is a literal)

"Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples."

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

http://purl.obolibrary.org/obo/PO_0009005

PO_0009005

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

http://purl.obolibrary.org/obo/PO_0025034

PO_0025034

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_1

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

http://www.ebi.ac.uk/efo/EFO_0000992

EFO_0000992

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

http://www.w3.org/1999/02/22-rdf-syntax-ns#type

type

http://purl.org/net/p-plan#Step

Step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

http://purl.obolibrary.org/obo/PO_0009005

PO_0009005

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

http://purl.obolibrary.org/obo/PO_0025034

PO_0025034

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_2

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

http://www.ebi.ac.uk/efo/EFO_0000992

EFO_0000992

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_3

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_4

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

http://purl.obolibrary.org/obo/CHEBI_17997

CHEBI_17997

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#_5

http://www.ebi.ac.uk/efo/EFO_0000992

EFO_0000992

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"shoot"

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion The assertion above http://purl.org/pav/createdWith

createdWith

https://nanotate.bitsfetch.com

nanotate.bitsfetch.com

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-05T11:34:30.681034"

(xsd:dateTime) .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/iannotate

iannotate

This is the identifier for this whole nanopublication. http://purl.org/np/RAysi2SPEK... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#step

step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#sig

sig

http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAysi2SPEK... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#sig

sig

http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal)

"RSA"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#sig

sig

http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal)

"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAysi2SPEK...#sig

sig

http://purl.org/nanopub/x/hasSignature has the signature (this is a literal)

"eSiNGrBxHqXmxmmCqrlvmweZZTeW3VTUrw2lTNwq4K2WW1chx/PJ3CRcPlaXad5Wic4dQlwR/7H4c84jKVZo8IUwjwr2r6Xz48Ed9NE+3sVFVVzxf9PySoK8MkasyqBAHRrxOqL3wBaT9oNSczexTwaTkFveF3RvjMPANeaE7+Q="

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/3HMP7H3GEeuJHqtY8IH5vg

3HMP7H3GEeuJHqtY8IH5vg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/8vHAnH3GEeu_64_J_NTjhg

8vHAnH3GEeu_64_J_NTjhg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/AsPl1n3HEeuSaoMKfzNR1A

AsPl1n3HEeuSaoMKfzNR1A

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/U4k0VH3GEeuKIutEUEKDNA

U4k0VH3GEeuKIutEUEKDNA

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/bwzvDn3GEeutc5OnvN9V8Q

bwzvDn3GEeutc5OnvN9V8Q

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/hB9uOn3GEeu1xVt7eD6q9A

hB9uOn3GEeu1xVt7eD6q9A

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/mxfzeH3GEeucLV_A-MvnNw

mxfzeH3GEeucLV_A-MvnNw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/rTnKzH3GEeuH5UcMOhvLdw

rTnKzH3GEeuH5UcMOhvLdw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAysi2SPEK...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/winXQn3GEeudQt97vsqqag

winXQn3GEeudQt97vsqqag

This is the identifier for this whole nanopublication. http://purl.org/np/RAysi2SPEK...

this nanopublication

http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-05T11:34:30.681034"

(xsd:dateTime) .

This is the identifier for this whole nanopublication. http://purl.org/np/RAysi2SPEK...

this nanopublication

http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/iannotate

iannotate

Nanotate, 5 Mar 2021, 11:34:30 UTC

References

Raw

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