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step

Full identifier: http://purl.org/np/RAYDwTtT3SWmPi5y6QPlgud6dumB_mJt6XQ3oYluNNiVU#step

Assigned to 1 class:

http://purl.org/net/p-plan#Step Step ( )

Minted in Nanopublication

RAYDwTtT3S Step comment approve/disapprove edit as derived nanopublication

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Nanotate, 24 Feb 2021, 15:57:29 UTC

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
http://purl.org/np/RAw7yeTOzB... RAw7yeTOzB	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/net/p-plan#isStepOfPlan isStepOfPlan	http://purl.org/np/RAw7yeTOzB...#plan plan	Nanotate	2021-02-24T16:10:21.737Z
http://purl.org/np/RAw7yeTOzB... RAw7yeTOzB	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	http://purl.org/np/RAMDd8-ZAR...#step step	Nanotate	2021-02-24T16:10:21.737Z
http://purl.org/np/RAw7yeTOzB... RAw7yeTOzB	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	http://purl.org/np/RAB6KCX8--...#step step	http://www.ontologydesignpatterns.org/ont/dul/DUL.owl#precedes precedes	step	Nanotate	2021-02-24T16:10:21.737Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.w3.org/1999/02/22-rdf-syntax-ns#type type	http://purl.org/net/p-plan#Step Step	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/dc/elements/1.1/description description	Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput	http://purl.org/np/RAYDwTtT3S...#_1 _1	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed	http://purl.org/np/RAYDwTtT3S...#_2 _2	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed	http://www.bioassayontology.org/bao#BAO_0010014 BAO_0010014	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed	http://purl.org/np/RAYDwTtT3S...#_3 _3	Nanotate	2021-02-24T15:57:29.331Z
http://purl.org/np/RAYDwTtT3S... RAYDwTtT3S	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed	http://purl.obolibrary.org/obo/CHEBI_8984 CHEBI_8984	Nanotate	2021-02-24T15:57:29.331Z
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