_4 (explore) | nanodash

_4

Full identifier: http://purl.org/np/RAL4fGVNElkRcbLg5AIKk4Er-9QrfJoeLRCd_PMRSXD7g#_4

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Minted in Nanopublication

RAL4fGVNEl Step comment approve/disapprove edit as derived nanopublication

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_1

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"mosquitoes"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_10

_10

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1% SDS"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_2

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1% Triton -X"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_3

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"mosquitoes"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_4

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"micropistle"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_5

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1.5 ml eppendorf tube"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_6

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1X STE buffer"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_7

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"Proteinase K (20mg/ml)"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_8

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"100mM sucrose"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_9

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"Rnase A (20mg/ml)"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

http://purl.org/dc/elements/1.1/description

description

(this is a literal)

"Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_1

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

http://www.w3.org/1999/02/22-rdf-syntax-ns#type

type

http://purl.org/net/p-plan#Step

Step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_3

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_4

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_5

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_10

_10

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_2

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_6

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_7

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_8

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#_9

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion The assertion above http://purl.org/pav/createdWith

createdWith

https://nanotate.bitsfetch.com

nanotate.bitsfetch.com

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-10T10:33:19.355370"

(xsd:dateTime) .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/alejandra.sandoval

alejandra.sandoval

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNEl... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#step

step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#sig

sig

http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNEl... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#sig

sig

http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal)

"RSA"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#sig

sig

http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal)

"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAL4fGVNEl...#sig

sig

http://purl.org/nanopub/x/hasSignature has the signature (this is a literal)

"Kd4Aa6fDdpMWhH77AgLNFo/GE7w50Y0bXMfwIOIVdP+2Jp7DcBZ3K28i9HOzqkwNBC9YZmAKwLlwHp818FZw1VIUbdMpPIaCSwdAsSJgsv/Ov/vuO6093NjHOignVj4QsE9+XO9WZOyFBX1XxDAbO2anaVMuoEQa4HYFefmlDOc="

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/8b4bAHJNEeu01RN1eV0q-Q

8b4bAHJNEeu01RN1eV0q-Q

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/ESqevnJOEeu1UX9lnqErIQ

ESqevnJOEeu1UX9lnqErIQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/IrIzfHJOEeujx7t3IvpXzA

IrIzfHJOEeujx7t3IvpXzA

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/OkmgznJOEeudNEPmE26k-Q

OkmgznJOEeudNEPmE26k-Q

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/UBFH9HJOEeusy1eAko5oQw

UBFH9HJOEeusy1eAko5oQw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/Usa7nHJNEeuoHBdiYKYtlg

Usa7nHJNEeuoHBdiYKYtlg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/cWBYjnJMEeuOKD-DjOC3Cw

cWBYjnJMEeuOKD-DjOC3Cw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/drQGkHJNEeudQGs6yWujow

drQGkHJNEeudQGs6yWujow

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/lG67TnJNEeu01GMwoWiMRA

lG67TnJNEeu01GMwoWiMRA

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAL4fGVNEl...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/rawtGHJMEeu0J4cNJ9O11g

rawtGHJMEeu0J4cNJ9O11g

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNEl...

this nanopublication

http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-10T10:33:19.355370"

(xsd:dateTime) .

This is the identifier for this whole nanopublication. http://purl.org/np/RAL4fGVNEl...

this nanopublication

http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/alejandra.sandoval

alejandra.sandoval

Nanotate, 10 Mar 2021, 10:33:19 UTC

References

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