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_1

Full identifier: http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_1

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Minted in Nanopublication

RA3MmCHHYD Step comment approve/disapprove edit as derived nanopublication

http://purl.obolibrary.org/obo/CHEBI_17992

CHEBI_17992

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"100mM sucrose"

http://purl.obolibrary.org/obo/CHEBI_29234

CHEBI_29234

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1% Triton -X"

http://purl.obolibrary.org/obo/CHEBI_8984

CHEBI_8984

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1% SDS"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_1

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"mosquitoes"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_2

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"mosquitoes"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_3

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"micropistle"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_4

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"Rnase A (20mg/ml)"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_5

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1X STE buffer"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_6

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"Proteinase K (20mg/ml)"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

http://purl.org/dc/elements/1.1/description

description

(this is a literal)

"Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it."

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

http://purl.org/net/SMARTprotocol#hasExperimentalInput

hasExperimentalInput

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_1

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

http://www.w3.org/1999/02/22-rdf-syntax-ns#type

type

http://purl.org/net/p-plan#Step

Step

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_2

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_3

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

http://www.bioassayontology.org/bao#BAO_0010014

BAO_0010014

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

http://purl.obolibrary.org/obo/CHEBI_17992

CHEBI_17992

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

http://purl.obolibrary.org/obo/CHEBI_29234

CHEBI_29234

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

http://purl.obolibrary.org/obo/CHEBI_8984

CHEBI_8984

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_4

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_5

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#_6

http://www.bioassayontology.org/bao#BAO_0010014

BAO_0010014

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"1.5 ml eppendorf tube"

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion The assertion above http://purl.org/pav/createdWith

createdWith

https://nanotate.bitsfetch.com

nanotate.bitsfetch.com

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-16T16:48:04.108013"

(xsd:dateTime) .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/iannotate

iannotate

This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#step

step

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig

sig

http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig

sig

http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal)

"RSA"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig

sig

http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal)

"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"

This is a local identifier minted within the nanopublication. http://purl.org/np/RA3MmCHHYD...#sig

sig

http://purl.org/nanopub/x/hasSignature has the signature (this is a literal)

"aZd/AL+wAll/02gNVAmhv8QGPBCTs7otfD4kaMz6CzndlDewoaVZcCmCBsl0xltZZZisvKm9bu+mhYyIwYX+5z4L3rxcyLp7AxPMD7roKQ7W9/5gRzY5DSAJH9kE9Y6jZKoTkDGwyXty1qOddP/83ut8S3q0w1HrYrcGVrpP6OU="

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/8Y0HtIJ9EeuxMKftfvVUrw

8Y0HtIJ9EeuxMKftfvVUrw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/AHbk6IJ-EeuLn7c9eitXuQ

AHbk6IJ-EeuLn7c9eitXuQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/CWoxtIahEeueMafFr2xIRQ

CWoxtIahEeueMafFr2xIRQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/FBJa-oJ-EeuVON8E0bPEnQ

FBJa-oJ-EeuVON8E0bPEnQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/GGaOiIahEeuEzbud0kuutg

GGaOiIahEeuEzbud0kuutg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/JzPoLoJ-EeubeTMBRz-oHg

JzPoLoJ-EeubeTMBRz-oHg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/P9yYHIJ-Eeuk1J8X-v5LOg

P9yYHIJ-Eeuk1J8X-v5LOg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/VhkMtIJ-EeuaQsuiGJkaPw

VhkMtIJ-EeuaQsuiGJkaPw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/dCvMjIJ-EeuYqLMMrOutfQ

dCvMjIJ-EeuYqLMMrOutfQ

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RA3MmCHHYD...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/i-hYaIJ-EeuaRdNsg6w5Hg

i-hYaIJ-EeuaRdNsg6w5Hg

This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD...

this nanopublication

http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-16T16:48:04.108013"

(xsd:dateTime) .

This is the identifier for this whole nanopublication. http://purl.org/np/RA3MmCHHYD...

this nanopublication

http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/iannotate

iannotate

Nanotate, 16 Mar 2021, 16:48:04 UTC

References

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