The small RNA omrA directly binds to the ribosome binding site of csgF mRNA, preventing its translation under oxidative stress.

(unknown)

2026-01-11T18:19:00.000Z

In silico typing can be an indispensable tool for rapid genotyping of Coxiella genomic sequences

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2026-01-11T12:00:00.000Z

The strength of in silico typing methods relies significantly on the quality of the input sequence

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2026-01-11T12:00:00.000Z

CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants.

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2026-01-11T00:00:00.000Z

CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants.

(unknown)

2026-01-11T00:00:00.000Z

The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics.

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2026-01-11T00:00:00.000Z

The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics.

(unknown)

2026-01-11T00:00:00.000Z

The mock CoLoC-seq control experiment uses detergent-permeabilized membranes to detect false positives and distinguish genuine organellar protection from intrinsic RNase resistance.

(unknown)

2026-01-11T00:00:00.000Z

CoLoC-seq validation in human mitochondria identified 351 transcripts, with mtDNA-encoded transcripts showing 100 percent protection and nDNA-encoded transcripts showing rapid degradation, demonstrating the method's accuracy.

(unknown)

2026-01-11T00:00:00.000Z

The CoLoC-seq experimental protocol integrates organellar isolation, RNase gradient treatment, custom library preparation with selective sequencing of intact transcripts, and kinetics model-based bioinformatic analysis.

(unknown)

2026-01-11T00:00:00.000Z