RAMb6wb2si (explore)

RAMb6wb2si

Full identifier: http://purl.org/np/RAMb6wb2si75-zR2bDNpfg0lD_pWNleR9rBA4QJ64EfBo

Assigned to 1 class:

http://www.nanopub.org/nschema#Nanopublication Nanopublication ( )

Status

Nanopublication

RAMb6wb2si Step comment approve/disapprove edit as derived nanopublication

http://purl.obolibrary.org/obo/OBI_0400155

OBI_0400155

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"water bath"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#_1

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"suspension"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#_2

http://www.w3.org/2000/01/rdf-schema#label

label

(this is a literal)

"100 mg/ml lysozyme"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

http://purl.org/dc/elements/1.1/description

description

(this is a literal)

"Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath. Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield."

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

http://www.w3.org/1999/02/22-rdf-syntax-ns#type

type

http://purl.org/net/p-plan#Step

Step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed

bioSampleUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#_1

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed

labEquipmentUsed

http://purl.obolibrary.org/obo/OBI_0400155

OBI_0400155

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed

reagentUsed

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#_2

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion The assertion above http://purl.org/pav/createdWith

createdWith

https://nanotate.bitsfetch.com

nanotate.bitsfetch.com

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion The assertion above http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-10T11:18:25.462488"

(xsd:dateTime) .

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion The assertion above http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/alejandra.sandoval

alejandra.sandoval

This is the identifier for this whole nanopublication. http://purl.org/np/RAMb6wb2si... This nanopublication the nanopublication consists of an introduction of the given resource http://purl.org/nanopub/x/introduces introduces This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#step

step

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#sig

sig

http://purl.org/nanopub/x/hasSignatureTarget has as target This is the identifier for this whole nanopublication. http://purl.org/np/RAMb6wb2si... this nanopublication .

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#sig

sig

http://purl.org/nanopub/x/hasAlgorithm has the algorithm (this is a literal)

"RSA"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#sig

sig

http://purl.org/nanopub/x/hasPublicKey has the public key (this is a literal)

"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB"

This is a local identifier minted within the nanopublication. http://purl.org/np/RAMb6wb2si...#sig

sig

http://purl.org/nanopub/x/hasSignature has the signature (this is a literal)

"AVq49EuW85NpvUlB6CB8eVMe0Jr5EYEXDCQYm9p6fF1znfEISpbUsiRzXIgCwVWYoRJ3zshHUNexjRblahiYJOvvQnwongr8MNHjhUhFhqJjgXG8trQhZ2uL0DwPB3OzOSuI9rz03wCdTzRcSrr9OSHxoqY81B5G6SBcQpeGSG8="

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/EF-yuIFSEeuet6vjBV_k6Q

EF-yuIFSEeuet6vjBV_k6Q

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/JfaRoIFSEeu374uFEHtqAg

JfaRoIFSEeu374uFEHtqAg

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/K0yIKoG8EeujNmf67OBRmw

K0yIKoG8EeujNmf67OBRmw

This is the identifier for the assertion of this nanopublication. http://purl.org/np/RAMb6wb2si...#assertion

the assertion above

http://www.w3.org/ns/prov#wasDerivedFrom

wasDerivedFrom

https://hypothes.is/a/tmG8cIFREeuamj8DU21Bpw

tmG8cIFREeuamj8DU21Bpw

This is the identifier for this whole nanopublication. http://purl.org/np/RAMb6wb2si...

this nanopublication

http://www.w3.org/ns/prov#generatedAtTime

generatedAtTime

(this is a literal)

"2021-03-10T11:18:25.462488"

(xsd:dateTime) .

This is the identifier for this whole nanopublication. http://purl.org/np/RAMb6wb2si...

this nanopublication

http://www.w3.org/ns/prov#wasAttributedTo

wasAttributedTo

https://hypothes.is/users/alejandra.sandoval

alejandra.sandoval

Nanotate, 10 Mar 2021, 11:18:25 UTC

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
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Raw

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