step (explore) | nanodash

Login with ORCID

step

Full identifier: http://purl.org/np/RA6F1VxP7thp4zX-1raNBn2QGqYfK0d8XZO_-jUUWiqNE#step

Assigned to 1 class:

http://purl.org/net/p-plan#Step Step ( )

Minted in Nanopublication

RA6F1VxP7t Step comment approve/disapprove edit as derived nanopublication

.

.

.

.

.

.

.

.

.

.

.

.

.

(xsd:dateTime) .

.

.

.

.

.

.

.

.

.

.

.

(xsd:dateTime) .

.

Nanotate, 22 Mar 2021, 16:05:00 UTC

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://www.w3.org/1999/02/22-rdf-syntax-ns#type type	http://purl.org/net/p-plan#Step Step	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/dc/elements/1.1/description description	Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	http://purl.org/net/SMARTprotocol#hasExperimentalInput hasExperimentalInput	http://purl.org/np/RA6F1VxP7t...#_1 _1	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...RAFT-2020_07_23/#bioSampleUsed bioSampleUsed	http://purl.org/np/RA6F1VxP7t...#_2 _2	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...T-2020_07_23/#labEquipmentUsed labEquipmentUsed	http://purl.obolibrary.org/obo/OBI_0002488 OBI_0002488	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed	http://purl.obolibrary.org/obo/OBI_0000079 OBI_0000079	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step	https://bioschemas.org/profile...-DRAFT-2020_07_23/#reagentUsed reagentUsed	http://purl.org/np/RA6F1VxP7t...#_3 _3	Nanotate	2021-03-22T16:05:00.244Z
http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	links a nanopublication to its pubinfo http://www.nanopub.org/nschema#hasPublicationInfo pubinfo	http://purl.org/np/RA6F1VxP7t... RA6F1VxP7t	http://purl.org/nanopub/x/introduces introduces	step	Nanotate	2021-03-22T16:05:00.244Z

Raw

TriG(txt), JSON-LD(txt), N-Quads(txt), XML(txt)