step-library-prep (explore) | nanodash

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step-library-prep

Full identifier: http://purl.org/coloc-seq/step-library-prep

Assigned to 1 class:

http://schema.org/Thing Thing ( )

References

Nanopublication	Part	Subject	Predicate	Object	Published By	Published On
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://www.w3.org/1999/02/22-rdf-syntax-ns#type type	http://schema.org/Thing Thing	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://www.w3.org/2000/01/rdf-schema#comment comment	Excludes RNase-cleaved transcripts (5'-OH and 3'-PO4 incompatible with ligation)	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/name name	Custom Library Preparation	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/description description	Selective sequencing of intact (non-RNase-cleaved) transcripts	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	3'-adapter ligation to hydroxyl ends	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	5'-adapter ligation to phosphorylated ends	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	Cap removal (RNA 5'-pyrophosphohydrolase)	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	First-strand cDNA synthesis (M-MLV reverse transcriptase)	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	PCR amplification (15 cycles, high-fidelity polymerase)	(unknown)	2026-01-11T00:00:00.000Z
https://w3id.org/np/RALlmEPut1... RALlmEPut1	links a nanopublication to its assertion http://www.nanopub.org/nschema#hasAssertion assertion	step-library-prep	http://schema.org/substep substep	Size selection: 10-220 nt on agarose gel	(unknown)	2026-01-11T00:00:00.000Z
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